REGULAR ARTICLES
Cell Research (1995)5:1-7
© 1995
SIBS, CAS All rights reserved 1001-0602/95
Chromosomal localization of ribosomal DNA sequences in an apple
rootstock using a digoxygenin detection system
ZHU JIMEI , S E GARDINER
The Horticulture and Food Research Institute of New Zealand Limited
Mt Albert Research Centre,Private Bag 92 196, Auckland, Nes Zealand
Batchelar Research Centre, Private Bag 11 030, Palmerston North, Nes Zealand
A 6kb rDNA probe comprising the 18s coding plus spacer sequences has been
hybridized to the metaphase chromosomes of apple rootstock cultivar MM106
demonstrating the localization of ribosomal gene arrays in the vicinity
of the telomeric regions of the short arms of chromosomes 6 and 14. The
in situ results using digoxygenin labeling coupled to an alkaline phosphatase
immunoassay were confirmed by silver staining for NORs and nucleoli. This
study demonstrates the feasibility of molecular cytogenetic analysis of
very small chromosomes (1.0-2.7 um) of apple.
Keywords : Malus, chromosomes, in situ hybridization, karyotype
analysis, silver staining. |
