REGULAR ARTICLES

Cell Research (1995)5:9-24
© 1995 SIBS, CAS All rights reserved 1001-0602/95

Laser scanning fluorescence microscopic measurement of the movement of cleaving egg surface of Rana Amurensis

Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.

Correspondence:

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Abstract

By laser scanning fluorescence microscopy for quantitative measurement of fluorescence intensity changes on egg surface stained with fluoresein isothiocyanate during cleavage furrow extending forward, it was found that in area of presumptive cleavage furrow the scanning curve became V shape, indicating dark stripe appeared in that place. Then the fluorescence intensity increased at the place where the bottom of V shape had located, and the scanning curve turned to ^ shape, indicating single stripe was formed. While enhanced fluorescence appeared on the borders or ^ shape, an M shape curve was found, showing double stripe occurred. During the distance between two borders of M shape increasing from 50 um to 100um, a fluorescence peak came to sight in the middle or the M shape, which being the cleavge furrow bottom. The two lateral sides of furrow bottom with decreasing fluorescence were nascent membrane. At that time the curve became W shape. By the sides of cleavage furrow the stress folds became cospicous after double stripe stage, showing the stretching of the egg surface being increased. With our [31,33] and others [32] reports that polylysine could induce the appearance of nascent membrane and phytohemagglutinins could decrease or prevent the appearance or nascent membrane, we believed the idea of Schroeder [25] that increasing mechanical stress could initiate nascent membrane formation and thought that the stress lay to the out sides of cleavage furrow.
Keywords : cleavage, egg surface movement, initiating nascent membrane formation, laser scanning fluorescence microscopy, Rana Amurensis.