REGULAR ARTICLES
Cell Research (1995)5:35-45
© 1995
SIBS, CAS All rights reserved 1001-0602/95
TGF-B receptors in mouse ES-5 cells and their differentiated derivatives
SHI WEIKANG, JUN WU, LUXIA XU, HSIAOCHIEN TSUNG..
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
By radioreceptor binding studies with iodinated BGF-B1, it has been shown that an undifferentiated ES-5 cell expresses approximately 3270 receptors with a dissociation constant Kd=130pM, but after the induction of differentiation by retinoic acid and dBcAMP, the receptor number of a differentiated RA-ES-5 cell was increased about 80% and the Kd was also increased to 370pM. Furthermore, more direct evidence supporting the expression of TGF-B type I and type II receptors in both ES-5 and RA-ES-5 cells has come from dot blot hybridization of cellular mRNA with cDNA probes for type I and type II receptors. Meanwhile, mRNA expression level of types I and II receptors in RA-ES-5cells were higher than that in ES-5 cells. Down-regulation of TGF-B receptors with a significant decrease in the rate of cell proliferation in both cells, was found by employing a pretreatment with neutralizing antibody to TGF-B1. The possible role of receptors for TGF-B in cell differentiation is discussed here.
Keywords : mouse embryonic stem (ES) cells, TGF-B receptors, Down-regulation by TGF-B antibody, RA-induced differentiation.
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