REGULAR ARTICLES Cell Research (1995)5:135-142 Inhibition of invasiveness and expression of epidermal growth factor receptor in human colorectal carcinoma cells induced by retinoic acid. SUN BAODONG, JINDAN SONG. Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang 110001, China.
Human amniotic basement membrane (HABM) model and agarose drop explant method were used to investigate the effects of retinoic acid(RA) on the invasiveness and adhesiveness to the basement membrane, and the migration of a highly invasive human colorectal cancer cell line CCL229. Results showed that 5×106 MRA markedly reduced the in vitro invasiveness and adhesiveness to the HABM, and the migration of the CCL229 cells. In addition, to elucidate the relation between expression of epidermal growth factor receptor (EGFR) and the invasiveness of the colorectal carcinoma cells, two well-differentiated, but with different invasiveness colorectal cancer cell lines were compared at mRNA level for expression of EGFR by using EGFR cDNA probe labeled with digoxigenin (DIG). Expression of EGFR was shown to be markedly higher in the highly invasive CCL229 cells than that in the low invasive CX-1 cells. Furthermore, expression of EGFR in RA treated CCL229 cells gradually decreased with time, the level being the lowest on day 6 of the RA treatment.
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