REGULAR ARTICLES
Cell Research (1996)6:1-9
© 1996
SIBS, CAS All rights reserved 1001-0602/96
Construction of normal human IgE phage antibody library and the
screedning of the anti-trichosanthin IgE
ZAN HONG, MING YEH
Shanghai Institute of Cell Biology, Chinese Academy of Sciences,
Shanghai 200031, China
Allergen specific IgE response is the major cause of immediate hypersensitively.
However the number of IgE-priducing B cells and the amount of IgE , especially
the soecific IgE ,are not low, it greatly impedes the study of the allergic-specific
antibody responses. Here we report the construction of a normal huamn IgE
combinatorical libarary . The repetorie of IgE
VH genes and of k gene were separately amplified from normal human peripheral
blood lymphocytes fhrough RT-PCR,and were then constructed to form the phage
surface display humanFab(IgEVH) library. A plant protein allergen, trichosanthin(TCS),
was used to affinity-enrich and to secrren the anti-TCS phage HuFab clones
from the library. Human IgE(Fab) to TCS were detected.
Keywords : Phageantibody library , human IgE(Fab), human anti-trichosanthin
IgE |
