REGULAR ARTICLES
Cell Research (1996)6:23-30
© 1996
SIBS, CAS All rights reserved 1001-0602/96
Correlation between inhibition of calcium -dependent apoptosis
by cyclosporin A and calcium transportation in HL-60 cells
HUANG QI QING, MING FANG, HONG QING ZHANG, SHAO BAI XUE
Department of Biology, Beijing Normal University, Beijing 100875
, China.
Correspondence:
SHAO BAI XUE |
|
Both calcium ionophore A23187 and endoplasmic reticulum Ca2+- ATPase inhibitionr
thapsigargin (TG) could increase intracellular free calcium concentration
and induce apoptosis in some cell lines . In the present study, we found
thar HL-60 cells treated with A23187 (1µg/ml) for 4 h or with TG (0.5
ug/ml) for 2 h showed typical characteristics of apoptosis. Pretreatment
with nontoxic concentration of cyclosporin A(CsA) (1ug/ml) could block these
effects. Flow cytometric analysis of intracellular Ca2+ after staining with
fluo-3 AM showed that CsA did not prevent the increase of intracellular
calcium induced by A23187 or Tg, but it could maintain the high level of
intancellular Ca2+ for a long time . These results suggest that CsA may
prevent calcium- induced apoptosis by blocking the transportation of Ca2+
in HL-60cells.
Keywords : Cyclosporin A, calcium ionophore A23187, thapsigargin,
apoptosis, intracellular calcium. |
