REGULAR ARTICLES
Cell Research (1996)6:47-53
© 1996
SIBS, CAS All rights reserved 1001-0602/96
Effect of captopril or verapamil on intracellular sodium in cultured
vascular smooth muscle cells.
QI JIAN HUA, LU ZHUANG, JUN WANG, MIN LU, XIN MING WANG, ZHENG JUN JIN
Shanghai Institute of Cell Biology, Chinese Academy of Sciences,
Shanghai 200031, China
Department of Parmacology , Shanghai Second Medical University, Shanghai
200025, China.
The effects of captopril (Cap) and verapamil (Ver) alone and in combination
on intracellular Na+ concentration([Na+]i) in cultured aortic smooth muscle
cells (ASMC) of rabbits was evaluated by a direct measurement of [Na+] I
with fluorescent dye sodium-binding benzofuran isophthalate (SBFI) combined
with digital. [Na+]I in resulting cells was found to be 11.9±0.7
mmol/L. Angiotensin II (Ang-II, 0.1-10 umol/L) induced an increase of [Na+]I
in concentration-dependent manner. Ver(0.1-10 µmol/L) inhibited Ang-II
(1µmol/L)-induced increase in [Na+]I while Cap enhanced Ang-II-induced
increase in [Na+]I at 10µmol/L but not at 0.1-1µmol/L. Ver(0.1-1µmol/L)
abolished enhancement of Ang-II-induced increase in [Na+]I by Cap . Thus
, the inhibition of Cap-enhanced[Na+]I byVer may provide a new hypothesis
for the underlying molecular mechanism of synergistic effect of the combination
of Ca2+ antagonists and angiotensin coverting enzyme inhibitors in controlling
blood pressure.
Keywords : Cultured aortic smooth muscle cells , angiotensin
II, captopril, verapamil,sodium-binding benzofuran isophthalate. |
