REGULAR ARTICLES
Cell Research (1996)6:55-63
© 1996
SIBS, CAS All rights reserved 1001-0602/96
Can iverexpression of TCG-ß1 gene change the sex ratio
in transgenic mice
TSUNG HSIAO CHEN, CHIEN,JIE XU,LU XIA XU,XIU LAN LI,WEI KANG SHI, ZHEN YAO
Shanghai Institute of Cell Biology, Chinese Academy of Sciences ,
Shanghai 200031,China
Mouse TGF-ß1 gene was microinjected into male pronuclei of F2 Hybrid
fertilized eggs obtained by mating CSJLF1 and C57BL/6J inbred strains to
generate transgenic mice with over-expressed TGF--ß1 gene. The rate
of found production is 31% and Southern blot analysis of founder mice tail
DNAs gave an integrated to the chromosomes of transgenix mice and transmitted
to their progeny at a rate of 33% in the second generation. Dot blot analysis
of tail RNA of some transgenic mice indicated a moderate expression of the
transgene. The most interesting finding of the present work is the striking
deviation from the normal male: female sex ration in transgenic mice, with
an average ration of 6.7:1. The possible nature of the predominance of male
sex in transgenic mice overexpressing TGF-ß1 is discussed.
Keywords : Transgenix mice, over-expressioned TGF-ß1
gene, MIS, sex ration. |
