REGULAR ARTICLES
Cell Research (1996)6:66-73
© 1996
SIBS, CAS All rights reserved 1001-0602/96
Molecular cloning and primary sequence analysis of a gene encoding
a putative chitinase gene in Brassica oleracea var . capitata
TANG GUO QING, YONG YAN BAI,SHI WEI LOO
Shanghai Institute of Plant Physiology, Chinese Academy of Sciences
, 300 Frenglin Road Shanghai, China 200032
Chitinase which catalyzes the hydrolysis of the ß-1, 4-acetyl-D-glucosamine
linkages of the fungal cell wall polymer chitin , is involved in inducible
plants defense system. By construction of cabbage (Brassica oleracea var.
capitata) genomic library and screening the library with pRCH8,a probe of
rice chitinase gene fragment, a chitinase genomic sequence was isolated
. the complete nucleotide sequence of the putative cabbage chittnase gene
(cabch29) was determined, with its longest open reading frame (ORF) encoding
a polypeptide of 413 aa . This polypeptide consists of a 21 aa N-terminal
signal peptide, two chitin-binding domains different from those of other
classes of plant chitinases , anda catalytic domain. Homology analysis illustrated
that this cabch29 gene has 58.8% identity at the nucleotide level with the
pRCH8 ORF probe and has 50% identity at the amino acid level with the catalytic
domain of chitinase from bean, maize and sugar beet. Meanwhile several kinds
of cis-elements, such as TATA box,CAAT box, GATA motif, ASF-1 binding site,
would-response elements and AATAAA,have also been discovered in the flanking
region of cabch29 gene.
Keywords : Cloning, sequence, cabbage, chitinase gene. |
