REGULAR ARTICLES
Cell Research (1996)6:75-84
© 1996
SIBS, CAS All rights reserved 1001-0602/96
Functional properties of a chitinase promoter from cabbage (Brassica
oleracea var. capital)
TANG GUO QING, YONG YAN BAI,SHI WEI LOO
Shanghai Institute of Plant Physiology, Chinese Academy of Sciences
, 300 Frenglin Road Shanghai, China 200032.
The 5’-region of the chitinase gene cabch29,derived from Brassica
oleracea var. capitata, has been sequenced and analyzed for cis-acting elements
important in controlling gene expression in transgenic tobacco plants .
Different 5’-deletion fragments were linked to reporter gene ß-glucuronidase
(GUS) as translational fusions, and the expression of the expression of
these chimeric genes was analyzed in vegetative organs and tissues. Sequences
up to-651 showed some basal GUS activity woth nearly equal levels in wounded
and intact tissues. The addition of further upstream sequences (-651 to
–1284) enhanced expression level , and the expression driven by this
fragment was inducible by a factor of two to three-fold by wounding . Histochemical
analysis of different tissue from transgenic plants that contain cabch29
promoter-GUS fusion gene demonstrated wound-inducible and tissue-specific
cabch29 promoter activity in plants containing the 1308 base pair fragment.
The location of GUS activity appears to be cells of stem, leaf and roots.
Meanwhile , the temporal and spatial expression of cabch29-GUS fusion gene
has been investigated . Among the different vegetative organs, a high level
of GUS activity was observed in stem and a moderate one on roots; whereas
, wounding stress led to a high level of GUS in wtem and moderate one in
leaf.
Keywords : Cabbage chitinase gene, promoter, cis-element,
would-response. |
