REGULAR ARTICLES
Cell Research (1996)6:137-144
© 1996
SIBS, CAS All rights reserved 1001-0602/96
Induction of apoptosis and change of bel-2 expression in macrophage
Ana-1 cells by all-trans retinoic aicd
YIN DE LING,XIU HAI REN,SHI ZHONG BU,YA LAN WU,LI ZHEN JIANG,ZHI JIANG WU,WEI
HU,GANG PEI
Shanghhai Institute of Cell Biology, Chinese Academy of Sciences,
Shanghai 200031, China
Macrophage cells play an important role in the initiation and regulation
of the immune response . All-transretinoic acid (ATRA) and its natural and
synthetic analogs (retinoids) affect a large number of biologyal processes.
Recently,retinoids have been shown promise in the therapy and prevention
of various cancers. However,many interesting questions related to the activities
of retinoids remain to be answered: (I) Molecular mechanisms by which retinoids
exert their effects; (II) shy the clinical uses of varying severity with
a higher frequency of blood system symptoms ;(III) little is known for its
impacts on macrophage cells etc. we set up this experiment ,therefore, to
examine the apoptosis of ATRA on macrophage Ana-1 cell line .Apoptosis of
the cells was quantitated after staining cells with propidium iodide (PI)
,by both accounting nuclear condensation and flow cytometry. When the cells
were treated with ATRA at or higher htan 1 µM for more than 24h ,sighificant
amount of the apoptotic cells was observed . Induction of apotosis of Ana-1
cells by ATRA was in time –and dose –dependent manners,exhibiting
the similar pattern as the apoptosis induced by actinomycin D (ACTD) . ATRA
treatment of Ana-a cells also caused the changes of the mRNA levels of ATRA-
induced apoptosis and change of bcl-2 in Ana-1 cells
Keywords : All-trans retinoic acid ,apoptosis,Ana-1 cells
bcl-2 |
