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Cell Research (1996)6:145-152
© 1996 SIBS, CAS All rights reserved 1001-0602/96

Antisense EGFR sequence enhances apoptosis in a human hepatoma cell line BEL-7404

Laboratory of Cellualr and Molecular Oncology ,Shanghai Institute of Cell Biology ,Chinese Academy of Sciences, Shanghai 200031
Shanghai Bureau of Hygiene
Laboratory Molecular Biology ,Naval Medical Research Institute ,Shanghai 200433,China

Correspondence: YONG HUA XU

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Abstract

Effects of antisense epidermal growth factor receptor (EGFR) sequence on apoptotic cell death were examined in a human hepatoma cell line BEL –7404 stably transfected with antisense EGFR vector (Cell Research ,2:75,1993) ,an enhanced rate (9.5%) of spontaneous apoptosis was detected by flow cytometry, whereas the rates of spontaneous apoptosis in JX-0 cells ,a sub-clone of BEL –7404 transfected by control vector ,and the parent BEL-7404 cells were almost equal and about 1.7%. Serum-starvation for 72h increased the rate of apoptosis of JX –1 cells up tp 33.7% ,while JX –0 and BEL –7404 cells ,under the same condition , produced less than 5% of apoptotic cells .Observation with electron microscope demonstrated that condensation and fragmentation of chromatin and formation of apoptotic bodies often occurred in JX-1 cells ,especially during serumstarvation . These results ,combined with the data of DNA fragmentation Elisa test, suggested that antisense EGFR sequence enhances apoptosis in the human hepatoma cells.
Antisebse EGFR ebgabces apoptosis in epatoma cells

Keywords : Antisense EGFR ,hepatoma cells ,apoptosis.