REGULAR ARTICLES
Cell Research (1996)6:155-166
© 1996
SIBS, CAS All rights reserved 1001-0602/96
Resersion of malignancy in human gastric cancer MKN –45
cells through the transfection of transforming growth factor-ßtype
II receptor gene
SUN HONG,WEI KANG SHI, ZHEN YAO
Shanghhai Institute of Cell Biology, Chinese Academy of Sciences,
Shanghai 200031, China
Human gastric cancer MKN-45 cells which are resisrant to TGR-ßtype
I and type III receptors ,but not type II receptors ,have been used as model
system to reconstitute these cancer cells with TGR-ßRII cDNA . The
results of these experiments indicated that the reexpression of TGF-ßRII
gene in MKN –45 cells can restore their sensitivity to TGF-ß
growth inhibition ,decrease their growth rate, reduce their cloning efficiency
in soft agar and tumorigenicity in nude mice in stable transfectants, in
comparison with their control MKN-45 cells ,Among different RII transfectants,
dheir defference in the changes of these parameters , as a result of the
regain of autocrine negative growth control by TGF-ß, is roughly proportional
to their level of expression of transfected RII mRNA . From these data ,
it is concleded that the inactivation of TGF-ß RII gene is related
to the excape of growth control by TGF-ß in MKN-45 cells . The importance
of the study of the interplay of TGF-ß and tis receptor system in
the nagative growth control of gastric cancer , and possibly also of other
cancers , is discussed.
Keywords : Gastric cancer cell, TGF-ß RII gene reconstitution
,growth inhibation , reduced tumorigenicity. |
