REGULAR ARTICLES
Cell Research (1996)6:189-199
© 1996
SIBS, CAS All rights reserved 1001-0602/96
Abrupt decrease of c-myc expression by antisense transcripts
induced terminal differerntiation and apoptosis in human promyelocytic
leukemuia HL-60 cells
HAO XIU JUAN,PEI HSIEN TANG, XIU SEN LI FEI ZI JIANG, YONG ZHI XI, NING
MAO ,DE LIU DU , MIN WU
Institute of Basis Medical Sciences ,Beijing 100850
National Laboratory of Molecular Oncology, Institute of Cancer Research
Beijing, Chinese Academy of Medical Sciences, Beijing 100021
Correspondence:
PEI HSIEN TANG |
|
This study was designed using c-myc antisense transcripts to evaluate how
alteration of c-myc expression in human myeloid leukemic HL-60 cells could
influence the myelomonocytic differentiation and induction of apoptosis.
The recombinant plasmid pDACx expressing antisense transcripts to c-myc
fragment containing a part of inron 1 and 137 nt exon 2 was constructed.
PDACx was transfected into HL –60 cell line by lipofectin , peroxidase
and a-NAE as well as detection of CD13 and CD33 antigens by flow cytometric
analysis indicated occurrence of myelomonocytic differentiation in cells
expression antisense transcripts to c-myc. DNA degradation measured by DNA
gel electrophoresis and typical morphological changes observed under electron
microscope proved the switch –on of apoptosis in terminally differentatin
HL –60 cells.
Keywords : c-myc oncogene, antisense transcripts , differentiation
,apoptosis. |
