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Cell Research (1997)7:13-19
© 1997 SIBS, CAS All rights reserved 1001-0602/97

Human chromosome pellicle antibody recognizing centromere protein-C (CENP-C), the main component of the kinetochore.

Xie Y, Ni ZM, Gu JR, Wong P, Wu WQ, Xu GW.

Hong Kong University of Science and Technology, Department of Biology, Hong Kong
Correspondence:
Xie Y
E-mail:boyxie@usthk.ust.hk
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Xie Y

Abstract
Recently the antichromosome antisera from several scleroderma patients have been found to recognize the pellicle of metaphase and anaphase chromosomes. In order to identify the pellicle components, we used these antichromosome antisera to screen a human embryonic cDNA library. The sequences of the positive clones are identical to the cDNA gene sequence of CENP-C (centromere protein C), a human centromere autoantigen. This result suggests that CENP-C is a component of the pellicle of human metaphase and anaphase chromosomes.

Keywords : Human antibody, scleroderma, CENP-C(centromere protein C), metaphase, chromosome pellicle, indirect immunofluorescent staining.

 

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