REGULAR ARTICLES
Cell Research (1997)7:31-38
© 1997 SIBS, CAS All rights reserved 1001-0602/97
Cytological identification of an isotetrasomic in rice and its
application to centromere mapping.
Cheng ZK, Yu HX, Yan CJ, Zhu LH, Gu MH.
Department of Agronomy, Agriculture College, Yangzhou University.
Correspondence:
Cheng ZK
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Cheng ZK
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The aneuploid with isochromosome or telochromosome is ideal material for
exploring the position of centromere in lingkage map. For obtaining these
aneuploids in rice, the primary trisomics from triplo-1 to triplo-12 and
the aneuploids derived from a triploid of indica rice variety Zhongxian
3037 were carefully investigated. From the offsprings of triplo-10, a primary
trisomic of chromosome 10 of the variety, an isotetrasomic "triplo-10-1"
was obtained. Cytological investigation revealed that a pair of extra isochromosomes
of triplo-10-1 were come from the short arm of chromosome 10. In the offsprings
of the isotetrasomic, a secondary trisomic "triplo-10-2", in which
the extra- chromosome was an isochromosome derived from the short arm of
chromosome 10, was identified. With the isotetrasomic, secondary trisomic,
primary trisomic and diploid of variety Zhongxian 3037, different molecular
markers were used for exploring the position of the centromere of chromosome
10. Based on the DNA dosage effect, it was verified that the molecular markers
G1125, G333 and L169 were located on the short arm, G1084 and other 16 available
molecular markers were on the long arm of chromosome 10. So the centromere
of chromosome 10 was located somewhere between G1125 and G1084 according
to the RFLP linkage map given by Kurata et al[1]. The distance from G1125
to G1084 was about 3.2 cM.
Keywords : Rice,
isotetrasomic, centromere mapping. |
