REGULAR ARTICLES
Cell Research (1997)7:39-49
© 1997 SIBS, CAS All rights reserved 1001-0602/97
High efficiency DNA delivery into swine oocytes and embryos by
electronic pulse delivery (EPD).
Yang Y, Huang SH, Zhao X.
InCell, Inc., Santa Clara, California 95051, USA.
The production of transgenic swine for xenotransplantation has been proposed
as an optimal option to overcome the chronic shortage of human organ donors.
Generation of genetically engineered swine has been elusive due to the difficulties
in gene transfer. In order to achieve effective gene delivery, a key step
for the genetic modification, we applied electronic pulse delivery (EPD)
technology to introduce H2Kb-DC DNA construct into swine eggs. Using the
developed EPD Protocols, we have achieved good viability of the EPD treated
oocytes, satisfactory embryonic development of the EPD treated embryos,
and stable DNA transfer into the swine embryos with high efficiency. Thus,
application of the EPD technology promises to effectively facilitate the
generation of large transgenic mammals.
Keywords : Xenotransplantation,
transgenic pig, electronic pulse delivery (EPD), swine embryos, DNA transfer. |
