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Cell Research (1997)7:107-117
© 1997 SIBS, CAS All rights reserved 1001-0602/97
Association of DNA with nuclear matrix in in vitro assembled
nuclei induced by rDNA from Tetrahymena shanghaiensis in Xenopus egg extracts.
Chen Y, Zhang B, Li XF, Zhai ZH.
Department of Cell Biology and Genetics, College of Life Sciences,
Peking University, Beijing.
Correspondence:
Chen Y |
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Chen Y
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The nuclei assembled from exogenous DNA or chromatin in egg extracts resemble
their in vivo counterparts in many aspects. However, the distribution pattern
of DNA in these nuclei remains unknown. We introduced rDNA from the macronuclei
of Tetrahymena into Xenopus cell-free extracts to examine the association
of specific DNA sequences with nuclear matrix (NM) in the nuclei assembled
in vitro. Our previous works showed the 5'NTS (non-transcription sequences)
of the rDNA specifically bind to the NM system in the macronuclei. We show
now the rDNA could induce chromatin assembly and nuclear formation in Xenopus
cell-free system. When we extracted the NM system and compared the binding
affinity of different regions of rDNA with the NM system, we found that
the 5'NTS still hold their binding affinity with insoluble structure of
the assembled nuclei in the extracts of Xenopus eggs.
Keywords : Nuclear
assembly, nuclear matrix, Xenopus egg extracts, Tetrahymena rDNA. |
