ARTILCES

Cell Research (1997)7:161-170
© 1997 SIBS, CAS All rights reserved 1001-0602/97

Construction and diversity analysis of a murine IgE phage surface display library.

Shanghai Institute of Cell Biology, Chinese Academy of Sciences, China.

Correspondence: Yeh M E-mail: imceng@server.shcnc.ac.cn

Table of Contents Download as printable (PDF) file Full Text (html)file Search Medline for articles by: Yeh M

Abstract

To make further investigation of the IgE antibody repertoire in Trichosanthin (TCS) allergic responses, a murine IgE phage surface display library was constructed (3.0 x 10(5) independent clones). We first constructed the V epsilon cDNA library (4.6 x 10(5) independent clones) and V kappa cDNA library (3.0 x 10(5) independent clones). Then, the V epsilon and V kappa gene segments were amplified from both libraries by PCR respectively, and assembled into Fab fragment by SOE PCR. The phage library containing Fabs was thus constructed. The diversity of V epsilon from this library was analyzed and proved. Fab clones with high specificity to TCS have been screened out.

Keywords : Phage surface display library, trichosanthin, murine IgE antibody.