ARTICLES

Cell Research (1997)7:217-227
© 1997 SIBS, CAS All rights reserved 1001-0602/97

A preliminary analysis of antineoplastic activity of parvovirus MVMp NS-1 proteins.

Shanghai Institute of Digestive Diseases, Ren Ji Hospital, Shanghai Second Medical University, China.

Correspondence: Shen XZ

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Abstract

Human gastric cancer MKN-45 cells were transfected with pULB 3238, a plasmid carrying MVMp NS-1 gene with its original P4 promoter replaced by the glucocorticoid inducible promoter MMTV-LTR. After the integration and expression of NS-1 gene, some of the transfectants died, while others remained alive, but the growth features of survived cells were changed. For further study on the antineoplastic function of parvoviral NS-1 protein in vivo, transgenic mice carrying NS-1 genes were established by conventional method. Among 4 founders, one of them was found to be able to transmit the transgene to around 50% of their offsprings. RT-PCR was performed to indicate the expression of NS-1 gene in transgenic mice and its mRNA appeared in a variety of tissues. The expression of integrated NS-1 gene may correlate with the decreased incidence of tumor induced in vivo by chemical carcinogens.

Keywords : Autonomous parvovirus MVMp, nonstructural proteins, gene expression, transgenic mice, antineoplastic effect.