REGULAR ARTICLES
Cell Research (1998)8:119-134
© 1998 SIBS, CAS All rights reserved 1001-0602/98
An Arabidopsis embryonic lethal mutant with reduced expression
of alanyl-tRNA synthetase gene.
Ge SJ, Yao XL, Yang ZX, Zhu ZP.
Department of Plant Development, Shanghai Institute of Plant Physiology,
Chinese Academy of Sciences, China.
Correspondence:
Zhu ZP
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Zhu ZP
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In present paper, one of the T-DNA insertional embryonic lethal mutant of
Arabidopsis is identified and designated as acd mutant. The embryo development
of this mutant is arrested in globular stage. The cell division pattern
is abnormal during early embryogenesis and results in disturbed cellular
differentiation. Most of mutant embryos are finally degenerated and aborted
in globular stage. However, a few of them still can germinate in agar plate
and produce seedlings with shorter hypocotyl and distorted shoot meristem.
To understand the molecular basis of the phenotype of this mutant, the joint
fragment of T-DNA/plant DNA is isolated by plasmid rescue and Dig-labeled
as probe for cDNA library screening. According to the sequence analysis
and similarity searching, a 936 bp cDNA sequence (EMBL accession #: Y12555)
from selected positive clone shows a 99.8% (923/925bp) sequence homology
with Alanyl-tRNA Synthetase (AlaRS) gene of Arabidopsis thaliana. Furthermore,
the data of in situ hybridization experiment indicate that the expression
of AlaRS gene is weak in early embryogenesis and declines along with globular
embryo 'development' in this mutant. Accordingly, the reduced expression
of AlaRS gene may be closely related to the morphological changes in early
embryogenesis of this lethal mutant.
Keywords : AlaRS
gene, embryonic mutant, Arabidopsis. |
