REGULAR ARTICLES
Cell Research (1998)8:135-142
© 1998 SIBS, CAS All rights reserved 1001-0602/98
Isolation of 24 novel cDNA fragments from microdissected human
chromosome band.
Zhang M, Yu L, Hu PR, Bi AD, Xia JH, Deng HX, Zhao SY.
Institute of Genetics, Fudan University, Shanghai, China.
Correspondence:
Yu L
|
 Table
of Contents
Download as printable
(PDF) file
Full Text (html)file
Search Medline for articles
by:
Yu L
|
The strategy of isolating the band-specific expression fragments from a
probe pool generated by human chromosome microdissection was reported. A
chromosome 14q24.3 band-specific single copy DNA pool was constructed based
on this probe pool. Using total DNA of the pool as probe to hybridize the
human marrow cDNA library, 68 primary positive clones were selected from
5 x 10(5) cDNA clones. Among these primary clones, 32 secondary clones were
obtained after second-round screening and designed as cFD14-1-32. Finally,
24 band-specific expression fragments were identified from these 32 positive
clones by DNA hybridization. Those band-specific clones can hybridize to
both 14q24.3 DNA and human genomic DNA but can't hybridize to 17q11-12 DNA.
Partial sequences of 13 fragments of them were sequenced and identified
as novel cDNA sequences, and these sequences were proved to have some homology
with known genes in NCBI database. Analysis of expression spectrum of cFD14-1
suggested that the cDNA fragments thus obtained should be used to isolate
the genes can not been cloned in 14q24.3 region.
Keywords : Probe
pool, chromosome 14q24.3, single copy DNA pool, expression sequences of
benes. |
