REGULAR ARTICLES
Cell Research (1998)8:143-149
© 1998 SIBS, CAS All rights reserved 1001-0602/98
Analysis of the 5' flanking sequence of the human norepinephrine
transporter gene.
Huang F, Fei J, Ma SK, Zhu LH, Liu ZP, Cai GQ, Ye ZC, Guo LH.
Shanghai Institute of Cell Biology, Chinese Academy of Sciences,
China.
Correspondence:
Fei J
|
 Table
of Contents
Download as printable
(PDF) file
Full Text (html)file
Search Medline for articles
by:
Fei J
|
The human norepinephrine transporter (NET) gene was cloned and structurally
analyzed. The far 5' fragment containing exon 1 (a non-coding exon) and
exon 2 was sequenced. The transcription start site of the gene in human
brain stem tissue was determined by primer extension analysis. It was found
that the gene could be transcribed from multiple starting points. The 5'
flanking sequence contains a proximal G-C rich region, one possible GSG
element and several SP1 sites. However it does not contain TATA box and
CAAT box motifs. Gel shift analysis with nuclear extracts from different
tissues of mouse shows that the G-C rich region may be involved in tissue
specific expression of the gene.
Keywords : Norepinephrine
transporter gene, primer extension, transcription regulation. |
