SHORT COMMUNICATION
Cell Research (1998)8:159-165
© 1998 SIBS, CAS All rights reserved 1001-0602/98
Antisense oligonucleotide to insulin-like growth factor II induces
apoptosis in human ovarian cancer AO cell line.
Yin DL, Pu L, Pei G.
Shanghai Institute of Cell Biology, Chinese Academy of Sciences,
China.
Correspondence:
Yin DL,
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Yin DL,
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The effects of antisense oligonucleotide to insulin-like growth factor II
(IGFII) to induce apoptosis in human ovarian cancer cells were evaluated.
antiproliferation effects of antisense to IGFII in ovarian cancer AO cells
were determined by 3H-thymidine incorporation. Apoptosis of the IGFII antisense-treated
cells was quantitated by both nuclear condensation and flow cytometry after
cells were stained with propidium iodide. IGFII antisense (4.5 microM) treatment
of 48 h maximally inhibited proliferation of AO cells. More than 25% of
IGFII antisense-treated cells (4.5 microM for 24 h) had undergone apoptosis,
whereas less than 3% of the cells were apoptotic in either IGFII sense-treated
cells or untreated cells. Antisense oligonucleotide to IGFII significantly
inhibited cell proliferation and induced apoptosis in human ovarian cancer
AO cell. These data suggest that IGFII may be a potential target in treatment
of ovarian cancer and antisense oligonucleotide to IGFII may serve as a
therapeutic approach.
Keywords : Insulin-like
growth factor II (IGFII), antisense oligonucleotide, apoptosis, human ovarian
cancer AO cells. |
