REGULAR ARTICLES
Cell Research (1998)8:195-207
© 1998 SIBS, CAS All rights reserved 1001-0602/98
Phosphorylation and biosynthesis of high molecular weight proteins
of tumor nuclear matrix.
Bazarnova TM, Buldyaeva TV, Filatova LS, Akopov SB, Zbarsky IB.
NK Koltzov Institute of Developmental Biology, Russian Academy of
Sciences, Moscow, Russia.
Correspondence:
Zbarsky IB. |
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Zbarsky IB.
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Our previous studies showed a predominance of high molecular weight protein
group in tumor nuclear matrices. Contrary to normal cells, proteins of this
group are preferentially phosphorylated. Phosphoproteins of hepatoma nuclear
matrix are selectively subjected to rapid proteolysis. By alkali treatment
and a monoclonal antibody against phosphotyrosyl residue the presence of
two high molecular weight bands of phosphotyrosyl-containing proteins was
detected in nuclear matrices of tumor but not of normal liver cells. High
molecular weight protein group of tumor nuclear matrices revealed also a
rapid turnover and preferential incorporation of labeled amino acids selectively
inhibited by chloramphenicol.
Keywords : Tumors,
nuclear matrix, phosphoproteins, phosphotyrosine, nuclear protein biosynthesis. |
