REGULAR ARTICLES
Cell Research (1998)8:231-240
© 1998 SIBS, CAS All rights reserved 1001-0602/98
Effects of laminin glycopeptides on metastasis-related behaviors
of cancer cells.
Jiang XN, Zhou RL, Zhang S.
Department of Cell Biology, Beijing Medical University, China.
Our previous reports have shown that laminin-glycopeptides (LN-GPs), the
total glycopeptides prepared from laminin (LN), can prevent the experimental
lung metastasis and liver metastasis of mouse cancer cells. In order to
explore the anti-metastatic mechanism of LN-GPs, we studied the effects
of LN-GPs on metastasis-related behaviors of cancer cells in vitro. LN-GPs
did not affect cell survival. However, LN-GPs inhibited cell attachment
and spreading of S180 cells on LN- and Matrigel-substrate in dose-dependent
and time-dependent manners. Moreover, inhibition of cell attachment and
spreading on Matrigel substrates were much greater on Matrigel substrate
than on LN substrate. In the presence of LN-GPs, S180 cells on LN substrate
changed from a flattened polygonal shape to a round one, the migration of
S180 cells on LN substrate decreased, and the number of a highly invasive
human pulmonary giant carcinoma PG cells invading Matrigel filter in a Boyden
chamber was reduced. LN-GPs thus have multiple inhibitory effects on cancer
metastasis-related behaviors.
Keywords : Laminin,
laminin glycopeptides, matrigel, cancer cells, cancer metastasis. |
