SHORT COMMUNICATION
Cell Research (1998)8:311-315
© 1998 SIBS, CAS All rights reserved 1001-0602/98
Val 70, Phe 72 and the last seven amino acid residues of C-terminal
are essential to the function of norepinephrine transporter.
Liu YH, Huang F, Fei J, Zhao JX, Gu QB, Schwarz W, Guo LH.
Shanghai Institute of Cell Biology, Chinese Academy of Sciences,
China.
Correspondence:
Guo LH. |
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Guo LH.
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The norepinephrine transporter(NET) is a member of the Na+/Cl- dependent
neurotransmitter transporter family and constitutes the target of several
clinically important antidepressants. To delineate the critical amino acid
residues and the function of C-terminal in regulating transport activity
of NET, here we constructed two site mutants (V70F, F72V; V70I, F72V) and
one C-terminal truncated mutant (delta 611-617). The wild type and mutants
of NET were expressed in Xenopus oocytes by injection of their cRNA. We
found that all of these mutants lost their transport activity. These results
indicate that the amino acid residues of V70 and F72, and the last seven
amino acids of C-terminal are essential to the transport activity of NET.
Keywords : Norepinephrine,
transporter, neurotransmitter uptake, mutagenesis. |
