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SHORT COMMUNICATIONNi²⁺ treatment causes cement gland formation in ectoderm explants of Xenopus laevis embryo

HUANG Yong, Xiao Yan DING^*

Shanghai Institute of Cell Biology, Chinese Academy of Science, Shanghai 200031, China

ABSTRACT

We found T-type calcium channel blocker Ni²⁺ can efficiently induce the formation of cement gland in Xenopus laevis animal cap explants. Another T-type specific calcium channel blocker Amiloride can also induce the formation of cement gland, while L-type specific calcium channel blocker Nifedipine has no inductive effect. These results may offer us an new approach to study the differentiation of cement gland through the change of intracelluar calcium concentration.

Key words: Ni²⁺, cement gland, gastrulation, T-type calcium channel.

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