REGULAR ARTICLES

Cell Research (2000), 10, 71--80

Using a non-radioisotopic, quantitative TRAP-based me- thod detecting telomerase activities in human hepatoma cells

ZHANG Ru Gang, Xing Wang WANG, Jin Hui YUAN, Li Xia GUO, Hong XIE^*
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China

ABSTRACT
A non-radioisotopic, quantitative TRAP-based telomerase activity assay was established mainly by using SYBR Green-I staining instead of radioisotope. Comparing with conventional radioisotope based method, it was better in reproducibility and accuracy. Using this method, we found telomerase activities were absent in normal human liver cells, while detected in all of four human hepatoma cell lines (BEL-7404, SMMC-7721, QGY-7903 and HCCM) without significant differences.

Key words:Telomerase, non-radioisotopic telomerase assay, human liver cells, human hepatoma cells.

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