REGULAR ARTICLES  Cell Research (2001); 11(4):273-278

Auxin distribution and transport during embryogenesis and seed germination of Arabidopsis

NI Di An^1,#, Ling Jian WANG ¹, Chun Hong DING^2,#,#, Zhi Hong XU^1,3,*

¹Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
²College of Bioscience, East China Normal University, Shanghai 200062, China
³College of Life Sciences, Peking University, Beijing 100871, China
^#Present address: University of Potsdam, Institute of Biochemistry and Biology, AG Molecular-Biology,
  Karl-Liebknecht-Str. 24-25, Hause 20, Golm14476, Germany
^##Present address: Shanghai Experimental School of Elementary Education, Shanghai 201103, China

ABSTRACT

    Auxin distribution during embryogenesis and seed germination were studied with transgenic Arabidopsis plants expressing GUS gene driven by a synthetic DR5 promoter , an auxin responsive promoter. The results showed that GUS activity is higher in ends of hypophysis and cotyledon primordia of heart-, torpedo- and cotyledon-stage embryos, leaf tip area, lateral root primordia, root apex and cotyledon of young seedlings. And GUS accumulated in root apex of the seedlings grown on auxin transport inhibitor containing media. All these suggested that above-mentioned part of the organs and tissues have a higher level of auxin, and auxin polar transport inhibitor could cause the accumulation of auxin in root apex. And auxin transport inhibitor also resulted in aberration of Arabidopsis leaf pattern formation, root gravitropism and elongation.

Key words: Auxin, auxin distribution, auxin polar transport, auxin-responsive promoter, transgenic Arabidopsis.

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