REGULAR ARTICLES CellResearch (2002); 12(1):55-62

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Effects of cisplatin on telomerase activity and telomere length in BEL-7404 human hepatoma cells

Ru Gang ZHANG, Ru Ping ZHANG, Xing Wang WANG, Hong XIE^*

Department of Biotherapy, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China

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ABSTRACT

    Telomerase activity was inhibited in a dose and time-dependent manner with the treatment of cisplatin for 24, 48, or 72 h in a concentration ranged from 0.8 to 50 mM in BEL-7404 human hepatoma cells. There were no changes in expression pattern of three telomerase subunits, its catalytic reverse transcriptase subunit (hTERT), its RNA component (hTR) or the associated protein subunit (TP1), after cisplatin treated for 72 h with indicated concentrations. Mean telomere lengths were decreased by the cisplatin treatment. Cell growth inhibition and cell cycle accumulation in G₂/M phase were found to be correlated with telomerase inhibition in the present study, but percentages of cell apoptosis did not change markedly during the process.

Key words: Telomerase, telomere, cisplatin, hepatocellular carcinoma, cell cycle, cell growth.