REGULAR ARTICLES

Cell Research (2002); 12(5-6):401-405

Preparation and analysis of spermatocyte meiotic pachytene bivalents of pigs for gene mapping

Bang LIU, Geng Lin JIN, Shu Hong ZHAO, Mei YU, Tong An XIONG, Zhong Zhen PENG, Kui LI^*

Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China

Received Oct-22-2001 Revised Sep-15-2002 Accepted Oct-15-2002

Correspondence: Prof. Kui LI Tel: 0086-27-87281306,     Fax: 0086-27-87280408, E-mail: lkxblghi@public.wh.hb.cn

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Abstract

 Well-spread meiotic pachytene bivalents were obtained by using the prolonged hypotonic treatment combined with high chloroform Carnory's fixative solution from cells of the testes of domestic pigs. Comparison in the division index and length of pachytene bivalents with metaphase chromosomes showed that those of the former are 5 times higher and 3.42(1.87-5.98) times longer than those of the latter. Comparative studies on chromomere maps of bivalents and mitotic chromosomal G-bands were conducted by using the chromosome 12 as a example. Sex vesicle and various shapes of synaptic sex chromosomes have been observed. Two-color PRimed IN Situ (PRINS) labeling has been conducted successfully on pachytene bivalents of pigs.

Key words: pachytene bivalents, sex chromosome synapsis, two-color PRINS, microsatellites, pigs.