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Cell Research (2002); 12(5-6):407-410

Identification and characterization of a new member of serpin family- HongrES1 in rat epididymis

Zhi Hong HU, Qiang LIU, Quan SHANG, Min ZHENG, Jun YANG, Yong Lian ZHANG^*

State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China

Received Jun-13-2002  Revised Oct-24-2002  Accepted Oct-27-2002

Correspondence: Prof. Yong Lian ZHANG Tel: 0086-21-64374430-5293 Fax: 0086-21-64338357, E-mail:yonglz@sunm.shcnc.ac.cn

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Abstract

A full length cDNA named HongrES1 was isolated and cloned by screening rat epididymis cDNA library using a mouse EST as a probe and 5'RACE followed. It contained 1590bp nucleotides and its predicted protein had 415 amino acid residues including a serpin (serine protease inhibitor) conserved domain. Tissue distribution pattern showed it was specifically expressed in adult rat epididymis; moreover, in situ hybridyzation indicated this gene was expressed in a limited region of the cauda epididymis near vas deference. Such kind of expression pattern sugested that HongrES1 had potential function in male reproduction.

Key words: HongrES1, serpin, epididymis, a₁-antitrypsin, in situ hybridization.