REGULAR ARTICLES

Cell Research (2003); 13(2):83-91

Cloning and characterization of human IC53-2, a novel CDK5 activator binding protein

Yi Hu XIE¹, Xiang Huo HE¹, Yun Tian TANG², Jin Jun LI¹, Zhi Mei PAN¹, Wen Xin QIN¹, Da Fang WAN ^1*, Jian Ren GU^1*

¹State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032, China
²Peopele's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China

Received Oct-23-2002 Revised Dec-19-2002 Accepted Jan-9-2003

Correspondence: Da Fang WAN, Jian Ren GU 0086-21-64177401 (phone) 0086-21-64177401 (fax) nlorg@public.sta.net.cn

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We have identified IC53-2, a human homologue of the rat C53 gene from a human placenta cDNA library (GeneBank Accession No.AF217982). IC53-2 can bind to the CDK5 activator p35 by in vitro association assay. IC53-2 is mapped to human chromosome 17q21.31. The IC53-2 transcript is highly expressed in kidney, liver, skeletal muscle and placenta. It is abundantly expressed in SMMC-7721, C-33A, 3AO, A431 and MCF-7 cancer cell lines by RT-PCR assay. Stable transfection of IC53-2 cDNA into the hepatocellular carcinoma SMMC-7721 cell remarkably stimulates its growth in vitro. The above results indicate that IC53-2 is a novel human gene, which may be involved in the regulation of cell proliferation.

Key words: IC53-2, CDK5, p35, hepatocellular carcinoma, proliferation.