| REGULAR ARTICLES Cell Research (2003); 13(3):171-177 Expression and localization of VCX/Y proteins and their possible involvement in regulation of ribosome assembly during spermatogenesis Sheng Wei ZOU1, Jian Chao ZHANG2, Xiao Dong ZHANG1, Shi Ying MIAO1, Shu Dong ZONG2, Qi SHENG1, Lin Fang WANG1,* 1National Laboratory of Medical Molecular
Biology, Institute of Basic Medical Sciences, Chinese Academy of
Medical Sciences, Peking Union Medical College, 5 Dong Dan 3-tiao, Beijing
100005, China Received Dec-16-2002 Revised Mar-15-2003 Accepted Apr-8-2003
Variable Charge X/Y (VCX/Y) is a human testis-specific gene family that localized on X and Y chromosomes. In this study, VCY protein was expressed in E. coli in the form of glutathione-S-transferase (GST) fusion protein. With the purified fusion protein as antigen, the anti-GST-VCY antibody was generated and the localization of VCY protein in human testis was determined by immunohistochemistry. In the testis seminiferous epithelium, VCY proteins were highly expressed in nuclei of germ cells. Using propidium iodide staining and green fluorescent protein (GFP) tag technologies, VCY and VCX-8r proteins were mainly localized in the nucleoli of COS7 cells. In addition, the colocalization for VCY and VCX-8r in COS7 cells was also observed. With VCY cDNA as bait, a cDNA fragment of acidic ribosomal protein PO was obtained using yeast two-hybrid system. All the information above indicates that VCX/Y protein family might be involved in the regulation of ribosome assembly during spermatogenesis. Key words: VCX/Y protein, spermatogenesis, nucleoli, colocalization.
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