REGULAR ARTICLES

Cell Research (2003); 13(3):195-202

The culture and establishment of embryonic germ (EG) cell lines from Chinese mini swine

Hsiao Chien TSUNG^1,*, Zhong Wei DU^**, Rong RUI^***, Xiu Lan LI¹, Lin Ping BAO¹, Jun WU, Shi Min BAO², Zhen YAO¹

¹Laboratory of Embryonic Stem Cell, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biologcal Sciences, Chinese Academy of Sciences, Shanghai 200031, China
²Shanghai Laboratory Animal Center, Chinese Academy of Sciences, Shanghai 200233, China

Received Apr-14-2003 Revised May-16-2003 Accepted May-25-2003

^**Present address: Waisman center-University of Wisconsin T603, 1500 Highland Ave. Madison I 53705, USA. E-mail: zdu@wisc.edu
^*** Who had joined and contributed to the project in the early stage while holding a postdoctoral scholarship during 1997-99, now at the College of Veterinary Medicine, Nanking Agricultural University, Jiangsu 210095, China. E-mail: rrui@njau.edu.cn

Correspondence: Hsiao Chien TSUNG 0086-21-54921367 (phone) escg@sunm.shcnc.ac.cn

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As a part of a basic research project on Xeno-transplantion, we have been engaged in the derivation of embryonic stem cell lines from Chinese mini swine. Here, we reported for the first time the establishment of two porcine EG cell lines (BPEG1 and BPEG2) from primordial germ cells of genital ridges of a 28 and a 27 d embryos respectively. Their pluripotent nature has been identified by colony morphology, marker characterization as well as by in vitro and in vivo differentiation. These porcine EG cells are potentially useful for further basic studies.

Key words: primordial germ cells, EG cells, porcine, Chinese mini swine.