| |
 |
REGULAR ARTICLES
Cell Research (2003); 13(5):343-350 |
TGF-ß1-promoted epithelial-to-mesenchymal
transformation and cell adhesion contribute to TGF-ß1-enhanced cell
migration in SMMC-7721 cells
ZHEN XU1, MIN XIONG
SHEN2, DONG ZHU MA1, LI YING WANG1,
XI LIANG ZHA1*
1Key Laboratory of
Glycoconjugate Research, Ministry of Public Health, Department of Biochemistry
and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai
200032, China. Email: xlzha@shmu.edu.cn
2Medical School, Huzhou
Teacher's College, Huzhou Zhejiang 313000, China
*Correspondence:
Prof. Xi Liang ZHA, Tel: 0086-
021-54237696
Received May-13-2003
Revised July-28-2003 Accepted-Sept-18-2003
ABSTRACT
Transforming growth factor-ß1 (TGF-ß1), a multi-function polypeptide, is a double-edged
sword in cancer. For some tumor cells, TGF-ß1 is a potent growth inhibitor and
apoptosis inducer. More commonly, TGF-ß1 loses its growth-inhibitory and apoptosis-inducing
effects, but stimulates the metastatic capacity of tumor cells. It is currently
little known about TGF-ß1-promoted cell migration in hepatocellular carcinoma
(HCC) cells, let alone its mechanism. In this study, we found that TGF-ß1 lost
its tumor-suppressive effects, but significantly stimulated cell migration in
SMMC-7721 human HCC cells. By FACS and Western blot analysis, we observed that
TGF-ß1 enhanced the expression of a5ß1
integrin obviously, and subsequently stimulated cell adhesion onto fibronectin
(Fn). Furthermore, we observed that TGF-ß1 could also promote SMMC-7721 cells
adhesion onto laminin (Ln). Our data also provided evidences that TGF-ß1 induced
epithelial-to-mesenchymal transformation (EMT) in SMMC-7721 cells. First, SMMC-7721
cells clearly switched to the spindle shape morphology after TGF-ß1 treatment.
Furthermore, TGF-ß1 induced the down-regulation of E-cadherin and the nuclear
translocation of ß-catenin. These results indicated that TGF-ß1-promoted cell
adhesion and TGF-ß1-induced epithelial-to-mesenchymal transformation might be
both responsible for TGF-ß1-enhanced cell migration.
Key words: TGF-ß1,
cell migration, epithelial-to-mesenchymal transformation, a5ß1 integrin.
Table of Contents
Download as printable (PDF) file -1.6MK
Full Text (html)file
|
