REGULAR ARTICLES

Cell Research (2003); 13(6):509-514

Gene expression profile favoring phenotypic reversion: a clue for mechanism of tumor suppression by NF-IL6 3'UTR

Ding Gan LIU^{1 ,*}, Qiu Hong JIANG¹, Yun Yi WEI¹, Li SUN¹, Bei Bei FU¹, Fu Kun ZHAO² , Qiong ZHOU ¹

¹State Key Laboratory of Molecular Biology and ²Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. E-mail: dgliu@sibs.ac.cn

Received Sept-6-2003 Revised Nov-27-2003 Accepted Nov-29-2003

　

Correspondence: Dr. Ding Gan LIU Tel: 021-54921135 E-mail: dgliu@sibs.ac.cn

Table of Contents Download as printable (PDF) file -832K Full Text (html)file Search Medline for articles by: Ding Gan LIU

Abbreviations: NF-IL6, nuclear factor for interleukin-6 expression. PKR, double-stranded RNA-dependent protein kinase. 3'UTR, 3'untranslated region.

Abstract

Transfection of cDNA in 3'untranslated region of human nuclear factor for interleukin-6 (NF-IL6 3'UTR) induced tumor suppression in a human hepatoma cell line. cDNA array analysis was used to reveal changes in gene expression profile leading to tumor suppression The results indicate that this suppression was not due to activation of dsRNA-dependent protein kinase, nor to inactivation of oncogenes; rather, all the changes in expression of known genes, induced by NF-IL6 3'UTR cDNA may be ascribed to the suppression of cellular malignancy. Therefore, our results imply that this 3'untranslated region may have played role of a regulator of gene expression profile.
Key words: cDNA array, NF-IL6, hepatoma, 3'UTR, tumor suppression.