| ARTICLE Cell Research, 15(2):120-126, Feb 2005 Dynamic distribution of Ser-10 phosphorylated histone H3 in cytoplasm of MCF-7 and CHO cells during mitosis Deng Wen LI, Qin YANG, Jia Tong CHEN, Hao ZHOU, Ru Ming LIU, Xi Tai HUANG* Department of Biochemistry and molecular biology, College of Life Sciences, Nankai University, Tianjin 300071, China Received, March 9, 2004 Revised, Nov 21, 2004 Accepted, Dec 20, 2004
The dynamic distribution of phosphorylated Histone H3 on Ser10 (phospho-H3) in cells was investigated to determine its function during mitosis. Human breast adenocarcinoma cells MCF-7, and Chinese hamster cells CHO were analyzed by indirect immunofluorescence staining with an antibody against phospho-H3. We found that the phosphorylation begins at early prophase, and spreads throughout the chromosomes at late prophase. At metaphase, most of the phospho-H3 aggregates at the end of the condensed entity of chromosomes at equatorial plate. During anaphase and telophase, the fluorescent signal of phospho-H3 is detached from chromosomes into cytoplasm. At early anaphase, phospho-H3 shows ladder bands between two sets of separated chromosome, and forms "sandwich-like structure" when the chromosomes condensed. With the cleavage progressing, the "ladders" of the histone contract into a bigger bright dot. Then the histone aggregates and some of compacted microtubules in the midbody region are composed into a "bar-like" complex to separate daughter cells. The daughter cells seal their plasma membrane along with the ends of the "bar", inside which locates microtubules and modified histones, to finish the cytokinesis and keep the "bar complex" out of the cells. The specific distribution and kinetics of phospho-H3 in cytoplasm suggest that the modified histones may take part in the formation of midbody and play a crucial role in cytokinesis. Keywords: mitosis, cytokinesis, midbody, Ser10 phosphorylated H3, microtubule complex. |
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ISSN:1001-0602(Print),1748-7838(Online);CN:31-1568
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