ARTICLE

Cell Research, 15(3):183-186, Mar 2005

Golgi localization and dynamics of hyaluronan binding protein 1 (HABP1/p32/C1QBP) during the cell cycle

Aniruddha SENGUPTA¹, Bhaswati BANERJEE¹, Rakesh K. TYAGI², Kasturi DATTA^1,2,*

¹Biochemistry Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi-110067, India
²Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India

Received, June 29, 2004   Revised, Aug 29, 2005   Accepted, Jan 25, 2005

Correspondence: Kasturi DATTA +91-11-26172438 or 26704327 (phone) +91-11-26717502 (fax) kdatta@mail.jnu.ac.in

Table of Contents Download as printable (PDF) file -102K Full Text (html)file Search Medline for articles by: Kasturi DATTA

Abstract

Hyaluronan binding protein 1 (HABP1) is a negatively charged multifunctional mammalian protein with a unique structural fold. Despite the fact that HABP1 possesses mitochondrial localization signal, it has also been localized to other cellular compartments. Using indirect immunofluorescence, we examined the sub-cellular localization of HABP1 and its dynamics during mitosis. We wanted to determine whether it distributes in any distinctive manner after mitotic nuclear envelope disassembly or is dispersed randomly throughout the cell. Our results reveal the golgi localization of HABP1 and demonstrate its complete dispersion throughout the cell during mitosis. This distinctive distribution pattern of HABP1 during mitosis resembles its ligand hyaluronan, suggesting that in concert with each other the two molecules play critical roles in this dynamic process.

Keywords: Hyaluronan Binding Protein 1 (HABP1), hyaluronan, sub-cellular localization, golgi, mitosis.