| ORIGINAL ARTICLE
Cell Research (2006)16:106-112
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The identification of a new actin-binding region in p57
Chang Zhen Liu1, Yong Chen1, Sen Fang Sui1
1Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembranes, Tsinghua University, Beijing 100084, China
The actin-binding protein p57 is a member of mammalian coronin-like proteins. The roles of this protein in phagocytic processes conceivably depend on its interactions with F-actin. Two regions, p571-34and p57111-204, were previously reported to be actin-binding sites. In this study, we found that the C-terminal region of p57, p57297-461, also possessed F-actin binding activity. Furthermore, the leucine zipper domain at the C-terminus of p57297-461 was essential for this actin-binding activity. The F-actin cross-linking assay revealed that the region contained in p57297-461 was sufficient to cross-link actin filaments. Our results strongly suggested that there was a new actin-binding region at the C-terminus of p57.
Cell Research (2006) 16:106-112 . doi:10.1038/sj.cr.7310014; published online 16 January 2006
Keywords: actin-binding protein, p57, actin cytoskeleton, leucine zipper |
