ARTICLE

Cell Research (2006): 916-922
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Heterotrimeric G protein α subunit is involved in rice brassinosteroid response

Lei Wang, Yun-Yuan Xu1, Qi-Bin Ma, Dan Li, Zhi-Hong Xu, Kang Chong

Correspondence: Kang Chong Tel: +86-010-62836517; Fax: +86-010-82594821 E-mail: chongk@ibcas.ac.cn Received 22 May 2006; revised 10 September 2006; accepted 18 October 2006

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Heterotrimeric G proteins are known to function as messengers in numerous signal transduction pathways. The null mutation of RGA (rice heterotrimeric G protein ¦Á subunit), which encodes the ¦Á subunit of heterotrimeric G protein in rice, causes severe dwarfism and reduced responsiveness to gibberellic acid in rice. However, less is known about heterotrimeric G protein in brassinosteroid (BR) signaling, one of the well-understood phytohormone pathways. In the present study, we used root elongation inhibition assay, lamina inclination assay and coleoptile elongation analysis to demonstrated reduced sensitivity of d1 mutant plants (caused by the null mutation of RGA) to 24-epibrassinolide (24- epiBL), which belongs to brassinosteroids and plays a wide variety of roles in plant growth and development. Moreover, RGA transcript level was decreased in 24-epiBL-treated seedlings in a dose-dependent manner. Our results show that RGA is involved in rice brassinosteroid response, which may be beneficial to elucidate the molecular mechanisms of G protein signaling and provide a novel perspective to understand BR signaling in higher plants.

Cell Research (2006) 16:916-922. doi: 10.1038/sj.cr.7310111; published online 21 November 2006

Keywords: heterotrimeric G protein, ¦Á subunit, d1 mutant, BR signaling, rice