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Cell Research (2006)16: 313-318
© 2006 IBCB, SIBS, CAS All rights reserved 1001-0602/06 $ 30.00
www.nature.com/cr

Knockdown of CD146 reduces the migration and proliferation of human endothelial cells

Yanyong Kang^{1, 3}, Fengcai Wang^{1, 2}, Jing Feng¹, Dongling Yang¹, Xu Yang³, Xiyun Yan¹

¹National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing 100101, China; 2Chemistry Department, Anyang teacher's College, Anyang 455000, China; 3College of Life Science, Central China Normal University, Wuhan 430079, China

Correspondence: Xiyun Yan Tel: +86-10-6488 8583; Fax: +86-10-6488 8584; E-mail: yanxy@sun5.ibp.ac.cn Received 12 Sep 2005; revised 1 Dec 2005; accepted 21 Dec 2005; published online 16 March 2006

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Our previous study has demonstrated that CD146 molecule is a biomarker on vascular endothelium, which is involved in angiogenesis and tumor growth. However the mechanism behind is not clear. Here we have for the first time developed a novel CD146 blockade system using CD146 siRNA to study its function on endothelial cells. Our data showed that CD146 siRNA specifically blocked the expression of CD146 on both mRNA and protein levels, leading to the significant suppression of HUVEC proliferation, adhesion and migration. These results demonstrate that CD146 plays a key role in vascular endothelial cell activity and angiogenesis, and CD146 siRNA can be used as a new inhibitor for anti-angiogenesis therapy.

Cell Research (2006) 16:313-318. doi:10.1038/sj.cr.7310039; published online 16 March 2006

Keywords: small interfering RNA, adhesion molecule CD146, vascular endothelial cell