ORIGINAL ARTICLE

Cell Research (2006)16: 622-631
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Inhibition of the α-mannosidase Man2c1 gene expression enhances adhesion of Jurkat cells

Li Qu,Ji Yu Ju,Shuang Ling Chen, Yan Shi, Zhi Guang Xiang, Yi Qun Zhou, Yun Tian, Yin Liu,Li Ping Zhu

*These authors contributed equally to this work. Correspondence: Li Ping Zhu Tel: +86-10-65296447; Fax: + 86-10-65296447; E-mail: zhulp@pumc.edu.cn Received 26 Dec 2005; revised 20 Feb 2006; accepted 11 Apr 2006

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Protein N-glycosylation plays very important roles in immunity and α-mannosidase is one of the key enzymes in Nglycosylation. This paper reports that inhibition of α-mannosidase Man2c1 gene expression enhances adhesion of Jurkat T cells. In comparison to the controls with normal expression of the enzyme, Jurkat cells with the inhibition of Man2c1 gene expression (AS cell) formed larger aggregates in culture, indicating an enhancement of adhesion between the cells. mRNA differential display analysis discovered up-regulation of several adhesion molecule genes in the AS cell. Because of the pivotal role played by CD54-LFA-1 interaction in immune cell interaction, this study focused on the contribution of enhanced expression of CD54 and LFA-1 to the enhanced adhesion of AS Jurkat cells. These facts, including increased binding of AS cells to ICAM-1-Fc, Mg2+ activation of the binding of AS cells to ICAM-1-Fc and enhanced aggregation of AS cells, together with the inhibiting effect of a blocking CD11a mAb on the binding to ICAM-1-Fc and aggregation of the cells demonstrate an important contribution of enhanced CD54-LFA-1 interaction to increased adhesion between AS cells. The enhanced CD54-LFA-1 interaction also resulted in increased adhesion between AS Jurkat T cells and Raji B cells. In addition, AS cells showed cytoskeletal rearrangement. The data imply a biological significance of MAN2C1 in T-cell functioning.

Cell Research advance online publication 23 May 2006; doi: 10.1038/sj.cr.7310065

Keywords: Man2c1, Jurkat T cell, adhesion, CD54-LFA-1, cytoskeleton