ORIGINAL ARTICLE

Cell Research (2006): 651-660
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Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment

Hong-Li Lian, Xin Yu, David Lane, Wei-Ning Sun, Zhang-Cheng Tang, Wei-Ai Su

Correspondence: Wei-Ai Su Tel: +86-21-54924245; Fax: +86-21-54924015; E-mail: zstressc@online.sh.cn Received 12 Nov 2005; revised 23 Jan 2006; accepted 17 Feb 2006

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Nanog is a recently discovered homeodomain transcription factor that sustains the pluripotency of embryonic stem (ES) cells and blocks their differentiation into endoderm. The murine F9 embryonal carcinoma cell line is a well-documented model system for endoderm cell lineage differentiation. Here, we examined the function of Nanog in F9 cell endoderm differentiation. Over-expression of Nanog returns the F9 cells to the early status of ES cells and represses the differentiation of primitive endoderm and parietal endoderm in F9 cells, whereas it has no effect on the differentiation of visceral endoderm. In contrast, the expression of C-terminal domain-truncated Nanog spontaneously promotes endoderm differentiation in F9 cells. These data suggest that Nanog is required to sustain the proper undifferentiated status of F9 cells, and the C-terminal domain of Nanog transduces the most effects in repressing primitive endoderm and parietal endoderm differentiation in F9 cells.

Cell Research advance online publication 6 Jun 2006; doi: 10.1038/sj.cr.7310067

Keywords: Nanog, F9 embryonal carcinoma cells, endoderm, differentiation.