ORIGINAL ARTICLE

Cell Research (2007): 783-791
© 2007 IBCB, SIBS, CAS All rights reserved 1001-0602/06 $ 30.00
www.nature.com/cr

Quantitative and qualitative in vitro analysis of the stem cell potential of hematopoietic cells purified from murine skeletal muscle

Celine Haond¹, Françoise Farace¹, Martine Guillier¹, Yann Lécluse¹, Frederic Mazurier², William Vainchenker¹ and Ali G Turhan¹

Correspondence: Ali G Turhan Division of Laboratory Hematology, University of Poitiers, Hopital Jean Bernard, 2 Rue de la Milétrie, BP577 86021 Poitiers, France Tel: +33-5-49444953; Fax: +33-5-49444095 E-mails: ali.turhan@univ-poitiers.fr, a.turhan@chu-poitiers.fr

Download as printable (PDF) file Full Text (html)file Search Medline for articles by: Celine Haond

The murine skeletal muscle contains hematopoietic stem cells, but this potential has so far not been studied quantitatively or qualitatively in vitro. To quantify the hematopoietic stem cell potential, we have used highly purified SP/CD45⁺ cells in long-term culture initiating cell (LTC-IC) assays. The SP/CD45⁺ cell population purified from murine muscle was found to have significant stem cell activity with an LTC-IC frequency of 1/640. Single-cell-sorted SP/CD45⁺ cells from muscle exhibited robust proliferative activity in vitro at day 16 (380-fold amplification), especially after culture with OP-9 layers that also support embryonic stem cells. Amplified cell populations originating from single cells exhibited multilineage differentiation ability with evidence of myeloid, lymphoid and NK cell markers. Thus, our results demonstrate that hematopoietic stem cells that can be quantified by LTC-IC assays exist in the murine skeletal muscle and show also for the first time, at the single-cell level, that these cells exhibit multilineage differentiation ability and major proliferative potential.

Cell Research (2007) 17:783–791. doi: 10.1038/cr.2007.74; published online 11 September 2007

Keywords: hematopoiesis, stem cells, muscle