ORIGINAL ARTICLE

Cell Research (2008): 1141-1150
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Estrogen deficiency leads to telomerase inhibition, telomere shortening and reduced cell proliferation in the adrenal gland of mice

Sharyn Bayne¹, Margaret EE Jones², He Li¹, Alex R Pinto¹, Evan R Simpson² and Jun-Ping Liu¹

Correspondence: He Li, Department of Immunology, AMREP, Monash Medical School, Commercial Road, Prahran, Victoria 3181, Australia. Tel: +61-3-99030715; Fax: +61-3-99030120 E-mail: he.li@med.monash.edu.au; Jun-Ping Liu, Department of Immunology, AMREP, Monash Medical School, Commercial Road, Prahran, Victoria 3181, Australia. Tel: +61-3-99030715; Fax: +61-3-99030120 E-mail: jun-ping.liu@med.monash.edu.au

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Estrogen deficiency mediates aging, but the underlying mechanism remains to be fully determined. We report here that estrogen deficiency caused by targeted disruption of aromatase in mice results in significant inhibition of telomerase activity in the adrenal gland in vivo. Gene expression analysis showed that, in the absence of estrogen, telomerase reverse transcriptase (TERT) gene expression is reduced in association with compromised cell proliferation in the adrenal gland cortex and adrenal atrophy. Stem cells positive in c-kit are identified to populate in the parenchyma of adrenal cortex. Analysis of telomeres revealed that estrogen deficiency results in significantly shorter telomeres in the adrenal cortex than that in wild-type (WT) control mice. To further establish the causal effects of estrogen, we conducted an estrogen replacement therapy in these estrogen-deficient animals. Administration of estrogen for 3 weeks restores TERT gene expression, telomerase activity and cell proliferation in estrogen-deficient mice. Thus, our data show for the first time that estrogen deficiency causes inhibitions of TERT gene expression, telomerase activity, telomere maintenance, and cell proliferation in the adrenal gland of mice in vivo, suggesting that telomerase inhibition and telomere shortening may mediate cell proliferation arrest in the adrenal gland, thus contributing to estrogen deficiency-induced aging under physiological conditions.

Cell Research (2008) 18:1141–1150. doi: 10.1038/cr.2008.291; published online 21 October 2008

Keywords: estrogen, telomerase, telomeres, cell proliferation, aromatase knockout mice