ORIGINAL ARTICLE

Cell Research (2010): 576-586
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The BUD2 mutation affects plant architecture through altering cytokinin and auxin responses in Arabidopsis

Xia Cui^1,*, Chunmin Ge^1,3,*, Renxiao Wang^1,4, Huanzhong Wang^1,5, Weiqi Chen², Zhiming Fu¹, Xiangning Jiang², Jiayang Li¹ and Yonghong Wang¹

Correspondence: Yonghong Wang, Tel: +86-10-64806008; Fax: +86-10-64883428 E-mail: yhwang@genetics.ac.cn *These two authors contributed equally to this work.

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The ratio of auxin and cytokinin plays a crucial role in regulating aerial architecture by promoting or repressing axillary bud outgrowth. We have previously identified an Arabidopsis mutant bud2 that displays altered root and shoot architecture, which results from the loss-of-function of S-adenosylmethionine decarboxylase 4 (SAMDC4). In this study, we demonstrate that BUD2 could be induced by auxin, and the induction is dependent on auxin signaling. The mutation of BUD2 results in hyposensitivity to auxin and hypersensitivity to cytokinin, which is confirmed by callus induction assays. Our study suggests that polyamines may play their roles in regulating the plant architecture through affecting the homeostasis of cytokinins and sensitivities to auxin and cytokinin.

Cell Research (2010) 20:576–586. doi:10.1038/cr.2010.51; published online 13 April 2010

Keywords: BUD2; shoot branching; auxin; cytokinin