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IS607 TnpB is a hypercompact RNA-guided DNA endonuclease

Hui Yang^1,* , Dinshaw J. Patel^2,*

¹Key Laboratory of RNA Innovation, Science and Engineering, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China
²Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
* Correspondence: Hui Yang(yanghui@sibcb.ac.cn)Dinshaw J. Patel(pateld@mskcc.org)

The Zhiwei Huang lab report on a hypercompact IS607 TnpB protein that naturally co-occurs with the conserved RAGATH-18 RNA, thereby identifying a new family of compact RNA-guided DNA endonucleases. Such an IS607 TnpB-based approach for programmable genome editing in both Escherichia coli and human cells, combined with its structural and target-adjacent motif divergence from earlier reported IS200/IS605 TnpB systems, highlights the diversity of the non-Cas genome editor toolbox.

https://doi.org/10.1038/s41422-024-00973-w

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