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One-step generation of semi-cloned zebrafish carrying a defined genetic modification

Yirui Ai^1,† , Shifeng Li^1,† , Siqi Liu^1,† , Jia Xu^1,† , Yunbin Zhang¹ , Siyu Zhou^1,2 , Zhou Zhou¹ , Jinsong Li^1,3,* , Yiping Li^1,*

¹Key Laboratory of Multi-cell Systems, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China
²Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China
³Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
^†These authors contributed equally: Yirui Ai, Shifeng Li, Siqi Liu, Jia Xu
* Correspondence: Jinsong Li(jsli@sibcb.ac.cn)Yiping Li(yipingli@sibcb.ac.cn)

https://doi.org/10.1038/s41422-026-01266-0

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